Protogen Consulting

Scientific Consulting & Services 

Fields of expertise: Physiology, Molecular Biology, Genetics, Proteomics, Chemistry/Biochemistry, Automation and High-Throughput Technology, Assay Development/Automation and Miniaturization, Crystallography, Microarray, Drug Discovery, and Pharmacology

·        Expert Witness/Litigation Support Services

·        Product Development/Product Evaluation

·        Experiment Design

·        Project Management

·        Applications Development (High-Throughput life science (Molecular Biology/Genetic/Crystallography, etc.) applications and target specific assays development)

·        Setting up collaborations for your applications

·        Setting up b-testing sites for your applications

·        Assay Miniaturization

 
Examples of applications developed:

1.      Automated High-Throughput Microdrop Crystallization Using Corning CrystalEX 96 Well Protein Crystallization Microplates.

2.      DNA Sequencing Automation with the Rolling Circle Amplification Procedure Using Precision Glass Syringes in High Throughput Liquid Handling Systems; 40-fold Reduction in Amplification Reaction Volumes

3.      High Throughput RNA Purification with Ambion RNAqueous Kits on the Apogent Discoveries Tango Liquid Handling System

4.      The Use of Precision Glass Syringes and a Non-Contact Microsolenoid Dispenser for the Production of High Throughput Low Density Arrays

5.      Setting Up High-Throughput, Low-Volume Sequencing and PCR Reactions Using an Automated System Equipped with Precision Glass Syringes and a Non-Contact Microsolenoid Dispenser

6.      PCR Purification with the Whatman^Ò 96-well PCR Cleanup Kit on the Apogent Discoveries Hydra^Ò and Tango^TM Liquid Handling Systems

7.      High-Throughput Electrophoresis Using the Robbins^® Tango^TM Automated Liquid Handling System and the Invitrogen^TM E-Gel^® 96 system

8.      High Throughput Plasmid DNA Purification with the Millipore Montágeä Plasmid Miniprep₉₆ Kit on the Apogent Discoveries PlateMate Plusâ and Tangoä Liquid Handlers

9.      High-throughput electrophoresis using automated liquid handling microdispensers

10.  High Throughput Plasmid DNA purification Using Precision Glass Syringes

11.  High-speed Hydra®-Plus-One System for Auto­mated High-Throughput Protein Crystallography

12.  Array production using the Hydra microdispenser and the Tango Liquid handling system

13.  RNA Fractionation by Ion-Pair Reversed Phase High Performance Liquid Chromatography for the construction of larger-size insert cDNA libraries

14.  RNA analysis by Ion-Pair Reversed Phase High Performance Liquid Chromatography

• Development of Validation and Certification Procedure for Instruments, Assays, Kits, and Molecular Biology products:        

1. Accuracy determination
2. Precision determination (via linear response curve and determination of the coefficient of variance)
3. PCR certification procedures for plastics used in the PCR/RT-PCR or any other amplification applications (RNase Test, DNase Test, PCR inhibitor Test, DNA test, Endotoxin test)

• Laboratory Automation

1. Determination of automation instrument(s) best suited for your specific High-Throughput applications. Laboratory automation is limited by what a specific instrument can accommodate. Let us determine which specific instrument has the capabilities to fulfill your automation and process requirements.
2. Determination of High-Throughput kit(s) best suited for your specific applications. Keeping in mind economical cost benefit issues, while shortening throughput times, we will determine which kit in the market fulfills your protocol requirements, providing the highest amount of precision and accuracy. We will also help you determine if your applications can be miniaturized to help you lower sample costs.  
   
   

• Marketing